DCTN5 Recombinant Rabbit Monoclonal Antibody [JE58-30]
cat.: HA722706
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JE58-30
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 20 kDa
Isotype: IgG
Immunogen: Recombinant protein within human DCTN5 aa 1-182 / 182.
Positive control: MCF7 cell lysate, 293T cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, human breast cancer tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Cytoplasm, cytoskeleton, Chromosome, centromere, kinetochore.
Recommended Dilutions:
  WB
  IHC-P
  IP
1:1,000
1:200
1-2μg/sample
Uniprot #: SwissProt: Q9BTE1 Human | Q9QZB9 Mouse
Entrez Gene: 308961 Rat
Alternative names: DCTN 5 DCTN5 DCTN5_HUMAN Dynactin 4 Dynactin 5 (p25) Dynactin subunit 5 Dynactin subunit p25 p25
Images
HA722706_1.jpg Fig1: Western blot analysis of DCTN5 on different lysates with Rabbit anti-DCTN5 antibody (HA722706) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: Mouse kidney tissue lysate (40 µg/Lane)

Predicted band size: 20 kDa
Observed band size: 20 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722706) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722706_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-DCTN5 antibody (HA722706) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722706) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722706_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-DCTN5 antibody (HA722706) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722706) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722706_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-DCTN5 antibody (HA722706) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722706) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722706_5.jpg Fig5: DCTN5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722706 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722706 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: MCF7 cell lysate (input)
Lane 2: HA722706 IP in MCF7 cell lysate
Lane 3: Rabbit IgG instead of HA722706 in MCF7 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute; ECL: K1802
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.