ACAP2 Recombinant Rabbit Monoclonal Antibody [JE65-03]
cat.: HA722712
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ACAP2 aa 681-730 / 778. |
| Positive control: | HeLa cell lysate, A549 cell lysate, LO2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human lung tissue, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Endosome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q15057 Human | Q6ZQK5 Mouse | Q5FVC7 Rat |
| Alternative names: | ACAP 2 ACAP2 ACAP2_HUMAN ANK repeat and PH domain-containing protein 2 Arf GAP with coiled coil ANK repeat and PH domain containing protein 2 Arf GAP with coiled coil ANK repeat and PH domains 2 Arf-GAP with coiled-coil ArfGAP with coiled coil ankyrin repeat and PH domains 2 CENT B 2 Centaurin beta2 Centaurin-beta-2 CENTB 2 CENTB2 Cnt b2 Cnt-b2 Cntb2 KIAA0041 Similar to yeast ZINC FINGER PROTEIN GCS1 |
Images
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Fig1:
Western blot analysis of ACAP2 on different lysates with Rabbit anti-ACAP2 antibody (HA722712) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: LO2 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: Mouse liver tissue lysate (40 µg/Lane) Lane 7: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ACAP2 antibody (HA722712) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722712) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACAP2 antibody (HA722712) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722712) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-ACAP2 antibody (HA722712) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722712) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.