TMEM16A Recombinant Rabbit Monoclonal Antibody [PSH06-56]
cat.: HA722720
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 114 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TMEM16A aa 1-333. |
| Positive control: | HT-29 cell lysate, HCT 116 cell lysate, BxPC-3 cell lysate, HT-29. |
| Subcellular location: | Apical cell membrane, Presynapse. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q5XXA6 Human |
| Alternative names: | ANO 1 ANO1 ANO1_HUMAN Anoctamin 1 Anoctamin 1 calcium activated chloride channel Anoctamin-1 Anoctamin1 Ca2+ activated Cl- channel Calcium Activated Chloride Channel Discovered on gastrointestinal stromal tumors protein 1 DOG 1 DOG1 FLJ10261 Membrane protein Oral cancer overexpressed 2 Oral cancer overexpressed protein 2 ORAOV 2 ORAOV2 TAOS 2 TAOS2 TMEM 16A TMEM16A Transmembrane protein 16A (eight membrane spanning domains) Transmembrane protein 16A Tumor amplified and overexpressed sequence 2 Tumor-amplified and overexpressed sequence 2 |
Images
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Fig1:
Western blot analysis of TMEM16A on different lysates with Rabbit anti-TMEM16A antibody (HA722720) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HCT 116 cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 114 kDa Observed band size: 150 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722720) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HT-29 (positive) and HeLa (negative) labeling TMEM16A with Rabbit anti-TMEM16A antibody (HA722720) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TMEM16A antibody (HA722720) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HT-29 cells labeling TMEM16A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722720, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.