Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH06-58]
cat.: HA722721
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH06-58 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 223 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MYH7 aa 1,250-1,300. |
| Positive control: | Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, human skeletal muscle tissue. |
| Subcellular location: | Cytoplasm, myofibril, sarcomere. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P12883 Human | Q91Z83 Mouse | P02564 Rat |
| Alternative names: | Beta myosin heavy chain cardiac muscle beta isoform CMD1S CMH1 MPD1 MYH7 MYH7_HUMAN Myhc slow MyHC-beta MyHC-slow MYHCB Myopathy, distal 1 Myosin heavy chain (AA 1-96) Myosin heavy chain 7 Myosin heavy chain Myosin heavy chain slow isoform Myosin heavy chain, cardiac muscle beta isoform Myosin, heavy chain 7, cardiac muscle, beta Myosin, heavy polypeptide 7, cardiac muscle, beta Myosin-7 Rhabdomyosarcoma antigen MU RMS 40.7A SPMD SPMM |
Images
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Fig1:
Western blot analysis of Slow Skeletal Myosin Heavy chain on different lysates with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 2: Mouse spleen tissue lysate (negative) (40 µg/Lane) Lane 3: Rat skeletal muscle tissue lysate (40 µg/Lane) Lane 4: Rat spleen tissue lysate (negative) (40 µg/Lane) Predicted band size: 223 kDa Observed band size: 223 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722721) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722721) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722721) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.