ATF6 Recombinant Rabbit Monoclonal Antibody [PSH06-59]
cat.: HA722722
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PSH06-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATF6 aa 1-350. |
| Positive control: | HeLa cell lysate, A431 cell lysate, HUVEC cell lysate, 293T cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse pancreas tissue lysate, Mouse kidney tissue lysate, Rat pancreas tissue lysate, Rat kidney tissue lysate, rat pancreas tissue, rat kidney tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P ChIP |
1:1,000 1:200-1:1,000 Use 0.5~2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: P18850 Human Entrez Gene: 226641 Mouse | 304962 Rat |
| Alternative names: | Activating transcription factor 6 alpha Activating transcription factor 6 ATF 6 ATF6 alpha ATF6 ATF6-alpha ATF6A ATF6A_HUMAN cAMP dependent transcription factor ATF 6 alpha cAMP-dependent transcription factor ATF-6 alpha Cyclic AMP dependent transcription factor ATF 6 alpha DKFZp686P2194 ESTM49 FLJ21663 Processed cyclic AMP dependent transcription factor ATF 6 alpha Processed cyclic AMP-dependent transcription factor ATF-6 alpha |
Images
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Fig1:
Western blot analysis of ATF6 on different lysates with Rabbit anti-ATF6 antibody (HA722722) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: HUVEC cell lysate Lane 4: 293T cell lysate Lane 5: HepG2 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse pancreas tissue lysate Lane 9: Mouse kidney tissue lysate Lane 10: Rat pancreas tissue lysate Lane 11: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 75 kDa Observed band size: 100-110 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722722) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-ATF6 antibody (HA722722) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722722) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ATF6 antibody (HA722722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 1μM Thapsigargin for 1 hour with ATF6 (HA722722) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.