KDM1 / LSD1 Recombinant Rabbit Monoclonal Antibody [JE04-60]
cat.: HA722723
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IP
Clonality: Monoclonal
Clone number: JE04-60
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 93 kDa
Isotype: IgG
Immunogen: Recombinant protein within
Positive control: Jurkat cell lysate, HCT 116 cell lysate, C2C12 cell lysate, COS-1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat, human colon tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:1,000
1:100
1:50-1:200
1-2μg/sample
Uniprot #: SwissProt: O60341 Human | Q6ZQ88 Mouse
Entrez Gene: 500569 Rat
Alternative names: Amine oxidase (flavin containing) domain 2 Amine oxidase, flavin containing, 2 AOF2 BHC110 BRAF35 HDAC complex protein BHC110 BRAF35-HDAC complex protein BHC110 BRAF35/HDAC complex, 110-kD subunit CPRF EC1 FAD binding protein BRAF35 HDAC complex, 110 kDa subunit Flavin-containing amine oxidase domain-containing protein 2 KDM 1 KDM1 Kdm1a KDM1A_HUMAN KIAA0601 LSD 1 LSD1 Lysine (K) specific demethylase 1 Lysine (K) specific demethylase 1A Lysine demethylase 1A Lysine specific histone demethylase 1 Lysine specific histone demethylase 1A Lysine-specific demethylase 1 Lysine-specific demethylase 1A Lysine-specific histone demethylase 1A
Images
HA722723_1.jpg Fig1: Western blot analysis of KDM1 / LSD1 on different lysates with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: HCT 116 cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Lane 5: Mouse spleen tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 93 kDa
Observed band size: 110 kDa

Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722723) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722723_2.jpg Fig2: Immunocytochemistry analysis of Jurkat cells labeling KDM1 / LSD1 with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722723_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722723) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722723_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722723) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722723_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722723) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722723_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-KDM1 / LSD1 antibody (HA722723) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722723) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722723_7.jpg Fig7: KDM1 / LSD1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722723 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722723 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: Jurkat cell lysate (input)
Lane 2: HA722723 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722723 in Jurkat cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 44 seconds; ECL: K1801
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.