Activin Receptor Type IIB / ACVR2B Recombinant Rabbit Monoclonal Antibody [JE33-52]
cat.: HA722725
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ACVR2B aa 159-208 / 512. |
| Positive control: | 293T cell lysate, K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13705 Human | P27040 Mouse | P38445 Rat |
| Alternative names: | Activin A receptor type IIB Activin receptor type 2B Activin receptor type IIB Activin receptor type-2B ActR IIB ACTR-IIB ActRIIB ACVR 2B ACVR2B AVR2B_HUMAN HTX4 MGC116908 |
Images
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Fig1:
Western blot analysis of Activin Receptor Type IIB / ACVR2B on different lysates with Rabbit anti-Activin Receptor Type IIB / ACVR2B antibody (HA722725) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat heart tissue lysate (40 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722725) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.