PDZK1 Recombinant Rabbit Monoclonal Antibody [JE37-70]
cat.: HA722729
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE37-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PDZK1 aa 470-519 / 519. |
| Positive control: | Caco-2 cell lysate, Human liver tissue lysate. |
| Subcellular location: | Membrane, Cell membrane. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q5T2W1 Human |
| Alternative names: | 1700023D20Rik 2610507N21Rik 4921513F16Rik AI267131 AI314638 AL022680 C terminal linking and modulating protein CAP70 CFTR associated protein of 70 kDa CFTR associated protein, 70-KD CFTR-associated protein of 70 kDa CLAMP D3Ertd537e Dietary Pi-regulated RNA-1 Diphor-1 mPDZK1 Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Na(+)/H(+) exchanger regulatory factor 3 Na/Pi cotransporter C-terminal-associated protein 1 Na/Pi cotransporter C-terminal-associated protein NaPi Cap1 NaPi-Cap1 NaPiCap1 NHERF 3 NHERF-3 NHERF3 NHRF3_HUMAN OTTHUMP00000015572 PDZ domain containing 1 PDZ domain containing protein 1 PDZ domain-containing protein 1 PDZD1 PDZK1 Sodium hydrogen exchanger regulatory factor 3 Sodium-hydrogen exchanger regulatory factor 3 |
Images
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Fig1:
Western blot analysis of PDZK1 on different lysates with Rabbit anti-PDZK1 antibody (HA722729) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 70 kDa Exposure time: Lane 1: 16 seconds; Lane 2: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722729) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
PDZK1 was immunoprecipitated from 0.2 mg Caco-2 cell lysate with HA722729 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722729 at 1/500 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Caco-2 cell lysate (input) Lane 2: HA722729 IP in Caco-2 cell lysate Lane 3: Rabbit IgG instead of HA722729 in Caco-2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 44 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.