TIE2 Recombinant Rabbit Monoclonal Antibody [PSH06-63]
cat.: HA722732
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 126 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TIE2 aa 1-769. |
| Positive control: | EA.hy926 cell lysate, EA.hy926. |
| Subcellular location: | Cell membrane, Cell junction, focal adhesion, Cytoplasm, cytoskeleton, Secreted. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q02763 Human |
| Alternative names: | Angiopoietin 1 receptor Angiopoietin-1 receptor CD202b CD202b antigen Endothelial tyrosine kinase Endothelium specific receptor tyrosine kinase 2 hTIE 2 hTIE2 Hyk p140 TEK Soluble TIE2 variant 1 Soluble TIE2 variant 2 Tek tek tyrosine kinase TEK tyrosine kinase endothelial tek tyrosine kinase, endothelial TIE 2 TIE2 TIE2_HUMAN Tunica interna endothelial cell kinase Tyrosine kinase with Ig and EGF homology domains 2 Tyrosine kinase with Ig and EGF homology domains-2 Tyrosine protein kinase receptor TEK Tyrosine protein kinase receptor TIE 2 Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 Venous malformations multiple cutaneous and mucosal VMCM 1 VMCM VMCM1 |
Images
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Fig1:
Western blot analysis of TIE2 on different lysates with Rabbit anti-TIE2 antibody (HA722732) at 1/1,000 dilution. Lane 1: EA.hy926 cell lysate Lane 2: HEK-293 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 126 kDa Observed band size: 150 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722732) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HEK-293 (left, negative) and EA.hy926 (right, positive) cells labeling TIE2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722732, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.