cGAS Recombinant Rabbit Monoclonal Antibody [PSH06-78]
cat.: HA722736
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human cGAS aa 101-522. |
| Positive control: | THP-1 cell lysate, HT-29 cell lysate, NK-92 cell lysate, Caco-2 cell lysate, HeLa cell lysate, A431 cell lysate, LoVo cell lysate, PANC-1 cell lysate. |
| Subcellular location: | Nucleus, Chromosome, Cell membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q8N884 Human |
| Alternative names: | C6orf150 cGAMP synthase cGAS CGAS_HUMAN cGMP Synthase Chromosome 6 open reading frame 150 Cyclic GMP-AMP synthase h-cGAS Hypothetical protein LOC115004 Mab 21 domain containing 1 Mab-21 domain-containing protein 1 MB21D1 MGC131892 MGC142166 MGC142168 OTTHUMP00000016743 OTTHUMP00000039330 protein MB21D1 Uncharacterized protein C6orf150 |
Images
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Fig1:
Western blot analysis of cGAS on different lysates with Rabbit anti-cGAS antibody (HA722736) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HT-29 cell lysate Lane 3: NK-92 cell lysate Lane 4: Caco-2 cell lysate Lane 5: HCT 116 cell lysate (low expression) Lane 6: HepG2 cell lysate (low expression) Lane 7: HeLa cell lysate Lane 8: A431 cell lysate Lane 9: LoVo cell lysate Lane 10: PANC-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722736) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.