NEMP1 Recombinant Rabbit Monoclonal Antibody [PSH06-66]
cat.: HA722738
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH06-66 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse NEMP1 aa 309-444. |
| Positive control: | Mouse spleen tissue lysate, Mouse skin tissue lysate, Mouse placenta tissue lysate, Rat spleen tissue lysate, Rat skin tissue lysate, HeLa transfected with mouse NEMP1. |
| Subcellular location: | Nucleus inner membrane, Nucleus envelope. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q6ZQE4 Mouse Entrez Gene: 679504 Rat |
| Alternative names: | Nuclear envelope integral membrane protein 1 NEMP1 KIAA0286 TMEM194 TMEM194A |
Images
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Fig1:
Western blot analysis of NEMP1 on different lysates with Rabbit anti-NEMP1 antibody (HA722738) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate (no heat) Lane 2: Mouse skin tissue lysate (hot lysis) Lane 3: Mouse placenta tissue lysate (70℃ heat) Lane 4: Rat spleen tissue lysate (no heat) Lane 5: Rat skin tissue lysate (hot lysis) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722738) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa transfected with or without mouse NEMP1 cells labeling NEMP1 with Rabbit anti-NEMP1 antibody (HA722738) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NEMP1 antibody (HA722738) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.