MED12 Recombinant Rabbit Monoclonal Antibody [PSH06-67]
cat.: HA722739
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 243 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MED12 aa 1,001-1,350. |
| Positive control: | A549 cell lysate, HeLa cell lysate, K-562 cell lysate, LNCaP cell lysate, Jurkat cell lysate, HCT 116 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Rat placenta tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q93074 Human | A2AGH6 Mouse Entrez Gene: 679693 Rat |
| Alternative names: | Activator recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component ARC240 CAG repeat protein 45 CAGH45 HOPA KIAA0192 MED12 MED12_HUMAN Mediator complex subunit 12 Mediator of RNA polymerase II transcription subunit 12 OPA containing protein OPA-containing protein Thyroid hormone receptor associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component TNRC11 Trap230 Trinucleotide repeat containing gene 11 protein Trinucleotide repeat-containing gene 11 protein |
Images
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Fig1:
Western blot analysis of MED12 on different lysates with Rabbit anti-MED12 antibody (HA722739) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: LNCaP cell lysate Lane 5: Jurkat cell lysate Lane 6: HCT 116 cell lysate Lane 7: Caco-2 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: C2C12 cell lysate Lane 10: PC-12 cell lysate Lane 11: Rat placenta tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 243 kDa Observed band size: 250 kDa Exposure time: 1 minutes 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722739) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.