Axl Recombinant Rabbit Monoclonal Antibody [PSH06-68]
cat.: HA722740
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-68 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 98 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Axl aa 1-451. |
| Positive control: | NCI-H1299 cell lysate, HeLa cell lysate, HUVEC cell lysate, COS-1 cell lysate, NCI-H1299. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P30530 Human |
| Alternative names: | Adhesion related kinase AI323647 Ark Axl AXL oncogene AXL receptor tyrosine kinase AXL transforming gene AXL transforming sequence/gene EC 2.7.10.1 JTK11 Oncogene AXL Tyro7 Tyrosine protein kinase receptor UFO Tyrosine-protein kinase receptor UFO UFO UFO_HUMAN |
Images
|
Fig1:
Western blot analysis of Axl on different lysates with Rabbit anti-Axl antibody (HA722740) at 1/2,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: HeLa cell lysate Lane 4: HUVEC cell lysate Lane 5: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 140 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722740) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of NCI-H1299 (positive) and Jurkat (negative) labeling Axl with Rabbit anti-Axl antibody (HA722740) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Axl antibody (HA722740) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of Jurkat (left, negative) and NCI-H1299 (right, positive) cells labeling Axl. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.