ATP13A2 Recombinant Rabbit Monoclonal Antibody [PSH06-69]
cat.: HA722741
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH06-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 129 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATP13A2 aa 485-900. |
| Positive control: | mouse hippocampus tissue, mouse cerebral cortex tissue, HeLa cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, C6 cell lysate, COS-1 cell lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Lysosome membrane, Late endosome membrane, Endosome, multivesicular body membrane, Cytoplasmic vesicle, autophagosome membrane. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:1,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: Q9NQ11 Human | Q9CTG6 Mouse Entrez Gene: 362645 Rat |
| Alternative names: | AT132_HUMAN Atp13a2 ATPase type 13A2 CLN12 FLJ26510 HSA9947 KRPPD PARK9 Probable cation transporting ATPase 13A2 Probable cation-transporting ATPase 13A2 Putative ATPase RP1-37C10.4 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig3:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. |
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Fig4:
Western blot analysis of ATP13A2 on different lysates with Rabbit anti-ATP13A2 antibody (HA722741) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: Neuro-2a cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: COS-1 cell lysate (20 µg/Lane) Predicted band size: 129 kDa Observed band size: 160 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722741) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ATP13A2 antibody (HA722741) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722741) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ATP13A2 antibody (HA722741) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722741) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ATP13A2 antibody (HA722741) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722741) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.