CSF-1-R Recombinant Rabbit Monoclonal Antibody [PSH06-70]
cat.: HA722742
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH06-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 109 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CSF-1-R aa 1-536. |
| Positive control: | RAW264.7 cell lysate, J774A.1 cell lysate, RAW264.7. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:100 |
| Uniprot #: | SwissProt: P09581 Mouse |
| Alternative names: | C FMS CD 115 CD115 CD115 antigen CFMS Colony stimulating factor 1 receptor Colony stimulating factor I receptor CSF 1 R CSF 1R CSF-1 receptor CSF-1-R CSF1 R CSF1R CSF1R_HUMAN CSFR EC 2.7.10.1 FIM 2 FIM2 FMS FMS proto oncogene FMS protooncogene HDLS M-CSF Receptor M-CSF-R Macrophage colony stimulating factor 1 receptor Macrophage colony stimulating factor I receptor Macrophage colony-stimulating factor 1 receptor McDonough feline sarcoma viral (v fms) oncogene homolog MCSFR Oncogen FMS Proto-oncogene c-Fms V-FMS McDonough feline sarcoma viral oncogen homolog, formerly |
Images
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Fig1:
Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA722742) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: J774A.1 cell lysate Lane 3: Neuro-2a cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 109 kDa Observed band size: 90-160 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722742) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of RAW264.7 (positive) and Neuro-2a (negative) labeling CSF-1-R with Rabbit anti-CSF-1-R antibody (HA722742) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSF-1-R antibody (HA722742) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.