cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71]
cat.: HA722743
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human cIAP1 aa 201-618. |
| Positive control: | HT-29 cell lysate, TF-1 cell lysate, HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, Jurkat cell lysate, A549 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, Rat testis tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q13490 Human | Q62210 Mouse Entrez Gene: 60371 Rat |
| Alternative names: | API 1 API1 Apoptosis inhibitor 1 Baculoviral IAP repeat containing 2 Baculoviral IAP repeat containing protein 2 Baculoviral IAP repeat-containing protein 2 BIRC 2 BIRC2 BIRC2_HUMAN C IAP1 C-IAP1 Cellular inhibitor of apoptosis 1 cellular inhibitor of apoptosis protein 1 cIAP 1 cIAP1 HIAP 2 HIAP-2 HIAP2 IAP 2 IAP homolog B IAP-2 IAP2 Inhibitor of apoptosis protein 2 MIHB NFR2 TRAF signalling complex protein RING finger protein 48 RNF 48 RNF48 TNFR2 TRAF signaling complex protein 2 TNFR2-TRAF-signaling complex protein 2 |
Images
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Fig1:
Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722743) at 1/2,000 dilution. Lane 1: HT-29 cell lysate (20 µg/Lane) Lane 2: TF-1 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: A549 cell lysate (20 µg/Lane) Lane 8: HEK-293 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse testis tissue lysate (40 µg/Lane) Lane 12: Mouse brain tissue lysate (40 µg/Lane) Lane 13: Rat testis tissue lysate (40 µg/Lane) Lane 14: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722743) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722743) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-cIAP1 KD cell lysate (10 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 78 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722743) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.