cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-72]
cat.: HA722744
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: PSH06-72
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG
Immunogen: Recombinant protein within human cIAP1 aa 201-618.
Positive control: TF-1 cell lysate, HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, Jurkat cell lysate, A549 cell lysate, HEK-293 cell lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, HT-29, NIH/3T3, PC-12.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:2,000
1:100-1:250
1:1,000
Uniprot #: SwissProt: Q13490 Human | Q62210 Mouse
Entrez Gene: 60371 Rat
Alternative names: API 1 API1 Apoptosis inhibitor 1 Baculoviral IAP repeat containing 2 Baculoviral IAP repeat containing protein 2 Baculoviral IAP repeat-containing protein 2 BIRC 2 BIRC2 BIRC2_HUMAN C IAP1 C-IAP1 Cellular inhibitor of apoptosis 1 cellular inhibitor of apoptosis protein 1 cIAP 1 cIAP1 HIAP 2 HIAP-2 HIAP2 IAP 2 IAP homolog B IAP-2 IAP2 Inhibitor of apoptosis protein 2 MIHB NFR2 TRAF signalling complex protein RING finger protein 48 RNF 48 RNF48 TNFR2 TRAF signaling complex protein 2 TNFR2-TRAF-signaling complex protein 2
Images
HA722744_1.jpg Fig1: Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722744) at 1/2,000 dilution.

Lane 1: TF-1 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: A549 cell lysate (20 µg/Lane)
Lane 7: HEK-293 cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722744) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722744_2.jpg Fig2: Immunocytochemistry analysis of HT-29 cells labeling cIAP1 with Rabbit anti-cIAP1 antibody (HA722744) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-cIAP1 antibody (HA722744) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722744_3.jpg Fig3: Immunocytochemistry analysis of NIH/3T3 cells labeling cIAP1 with Rabbit anti-cIAP1 antibody (HA722744) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-cIAP1 antibody (HA722744) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722744_4.jpg Fig4: Immunocytochemistry analysis of PC-12 cells labeling cIAP1 with Rabbit anti-cIAP1 antibody (HA722744) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-cIAP1 antibody (HA722744) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722744_5.jpg Fig5: Flow cytometric analysis of HT-29 cells labeling cIAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722744, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA722744_6.jpg Fig6: Flow cytometric analysis of NIH/3T3 cells labeling cIAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722744, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.