TAF1 Recombinant Rabbit Monoclonal Antibody [JE40-02]
cat.: HA722747
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 215 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P21675 Human | Q80UV9 Mouse Entrez Gene: 317256 Rat |
| Alternative names: | BA2R CCG 1 CCG1 CCGS Cell cycle G1 phase defect Cell cycle gene 1 protein Complementation of cell cycle block G1 to S DYT3 protein NSCL 2 NSCL2 OF p250 TAF 1 TAF(II)250 TAF1 RNA polymerase II TAF130 TAF145 TAF2A TAFII 250 TAFII250 TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD TATA box binding protein (TBP)-associated factor TBP associated factor 1 TBP associated factor 145 kDa TBP associated factor 250 kDa Transcription factor TFIID p250 polypeptide Transcription initiation factor TFIID 250 kDa subunit Transcription initiation factor TFIID subunit 1 YGR274C |
Images
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Fig1:
Western blot analysis of TAF1 on different lysates with Rabbit anti-TAF1 antibody (HA722747) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Predicted band size: 215 kDa Observed band size: 250 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722747) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TAF1 antibody (HA722747) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722747) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.