Hex Recombinant Rabbit Monoclonal Antibody [JE37-45]
cat.: HA722753
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE37-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | HeLa cell lysate, A549 cell lysate, MCF7 cell lysate, HT-29 cell lysate. |
| Subcellular location: | Nucleus, nuclear body, Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q03014 Human |
| Alternative names: | Hematopoietically expressed homeobox Hematopoietically-expressed homeobox protein HHEX HEX HHEX HHEX_HUMAN HMPH Homeobox hematopoietically expressed Homeobox protein HEX Homeobox protein PRH HOX11L PEN PRH PRHX Proline rich homeodomain containing transcription factor |
Images
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Fig1:
Western blot analysis of Hex on different lysates with Rabbit anti-Hex antibody (HA722753) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: MCF7 cell lysate Lane 4: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 32 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Hex was immunoprecipitated from 0.2 mg MCF7 cell lysate with HA722753 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722753 at 1/1,000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/100,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: HA722753 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of HA722753 in MCF7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.