beta Amyloid 1-42 Recombinant Rabbit Monoclonal Antibody [PSH06-81]
cat.: HA722754
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH06-81 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 4 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human beta Amyloid 1-42 peptide. |
| Positive control: | APP/PS1, 6-month mouse of AD brain tissue. |
| Subcellular location: | Cell membrane, Membrane, Perikaryon, Cell projection, growth cone, Membrane, clathrin-coated pit, Early endosome, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P05067 Human | P12023 Mouse |
| Alternative names: | A4 A4_HUMAN AAA ABETA ABPP AICD-50 AICD-57 AICD-59 AID(50) AID(57) AID(59) Alzheimer disease amyloid protein Amyloid intracellular domain 50 Amyloid intracellular domain 57 Amyloid intracellular domain 59 APP APPI Beta amyloid protein 42 Beta APP42 Beta-APP40 Beta-APP42 C31 Cerebral vascular amyloid peptide CVAP Gamma-CTF(50) Gamma-CTF(57) Gamma-CTF(59) PN-II PreA4 Protease nexin-II S-APP-alpha S-APP-beta |
Images
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Fig1:
Western blot analysis of beta Amyloid 1-42 on different peptides with Rabbit anti-beta Amyloid 1-42 antibody (HA722754) at 1/1,000 dilution. Lane 1: Human Aβ37 full length peptide (negative) Lane 2: Human Aβ38 full length peptide (negative) Lane 3: Human Aβ39 full length peptide (negative) Lane 4: Human Aβ40 full length peptide (negative) Lane 5: Human Aβ41 full length peptide (negative) Lane 6: Human Aβ42 full length peptide (positive) Lane 7: Human Aβ43 full length peptide (negative) Lane 8: Mouse Aβ42 full length peptide (positive) Lysates/proteins at 100 ng/Lane. Predicted band size: 4 kDa Observed band size: 4 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722754) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue with Rabbit anti-beta Amyloid 1-42 antibody (HA722754) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722754) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue (negative control) with Rabbit anti-beta Amyloid 1-42 antibody (HA722754) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722754) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded APP/PS1, 6-month mouse of AD brain tissue labeling beta Amyloid 1-42 with Rabbit anti-beta Amyloid 1-42 antibody (HA722754) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722754, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.