HEXB Recombinant Rabbit Monoclonal Antibody [PSH06-83]
cat.: HA722757
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH06-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HEXB aa 1-556. |
| Positive control: | HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, U-87 MG cell lysate, MCF7 cell lysate, Caco-2 cell lysate, NCI-H1299 cell lysate, human kidney tissue, human lung cancer tissue, HeLa. |
| Subcellular location: | Lysosome, Cytoplasmic vesicle, secretory vesicle, Cortical granule. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC IF-Tissue |
1:2,000 1:2,000 1:250 1:1,000 1:200-1:400 |
| Uniprot #: | SwissProt: P07686 Human |
| Alternative names: | Beta hexosaminidase beta chain Beta hexosaminidase subunit beta Beta N acetylhexosaminidase Beta-hexosaminidase subunit beta chain A Beta-N-acetylhexosaminidase subunit beta Cervical cancer proto oncogene 7 protein Cervical cancer proto-oncogene 7 protein ENC 1AS Epididymis luminal protein 248 HCC 7 HCC-7 HCC7 HEL 248 HEX B Hexb HEXB_HUMAN Hexosaminidase B (beta polypeptide) Hexosaminidase B Hexosaminidase subunit B HexosaminidaseB N acetyl beta glucosaminidase N-acetyl-beta-glucosaminidase subunit beta |
Images
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Fig1:
Western blot analysis of HEXB on different lysates with Rabbit anti-HEXB antibody (HA722757) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: U-87 MG cell lysate Lane 6: MCF7 cell lysate Lane 7: Caco-2 cell lysate Lane 8: NCI-H1299 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 30/60 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722757) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HEXB antibody (HA722757) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722757) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-HEXB antibody (HA722757) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722757) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling HEXB with Rabbit anti-HEXB antibody (HA722757) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HEXB antibody (HA722757) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of HeLa cells labeling HEXB. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722757, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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