CSN7b Recombinant Rabbit Monoclonal Antibody [PSH06-84]
cat.: HA722758
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH06-84
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CSN7b aa 215-264.
Positive control: 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, HL-60 cell lysate, HT-29 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9H9Q2 Human | Q8BV13 Mouse
Entrez Gene: 363273 Rat
Alternative names: COP9 Complex Homolog Subunit 7b COP9 Constitutive Photomorphogenic Homolog Subunit 7b COP9 Signalasome Subunit 7b COP9 signalosome complex subunit 7b COPS7B CSN7B_HUMAN JAB1 Containing Signalosome Subunit 7b JAB1-containing signalosome subunit 7b SGN7b Signalosome subunit 7b
Images
HA722758_1.jpg Fig1: Western blot analysis of CSN7b on different lysates with Rabbit anti-CSN7b antibody (HA722758) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HT-29 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C6 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: Mouse testis tissue lysate
Lane 11: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 30 kDa
Observed band size: 30 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722758) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.