| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CSN7b aa 215-264. |
| Positive control: | 293T cell lysate, HeLa cell lysate, Jurkat cell lysate, HL-60 cell lysate, HT-29 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H9Q2 Human | Q8BV13 Mouse Entrez Gene: 363273 Rat |
| Alternative names: | COP9 Complex Homolog Subunit 7b COP9 Constitutive Photomorphogenic Homolog Subunit 7b COP9 Signalasome Subunit 7b COP9 signalosome complex subunit 7b COPS7B CSN7B_HUMAN JAB1 Containing Signalosome Subunit 7b JAB1-containing signalosome subunit 7b SGN7b Signalosome subunit 7b |
|
Fig1:
Western blot analysis of CSN7b on different lysates with Rabbit anti-CSN7b antibody (HA722758) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HL-60 cell lysate Lane 5: HT-29 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C6 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse testis tissue lysate Lane 11: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722758) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |