Phospho-Met (Y1234/Y1235) Recombinant Rabbit Monoclonal Antibody [PSH06-90]
cat.: HA722765
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 156 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human Met aa 1,201-1,250. |
| Positive control: | HeLa cell lysate, HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate. |
| Subcellular location: | Membrane; Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P08581 Human |
| Alternative names: | AUTS9 c met D249 Hepatocyte growth factor receptor HGF receptor HGF/SF receptor HGFR MET Met proto oncogene Met proto oncogene tyrosine kinase MET proto oncogene, receptor tyrosine kinase Met proto-oncogene (hepatocyte growth factor receptor) Met proto-oncogene Met protooncogene MET_HUMAN Oncogene MET Par4 Proto-oncogene c-Met RCCP2 Scatter factor receptor SF receptor Tyrosine-protein kinase Met |
Images
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Fig1:
Western blot analysis of Phospho-Met (Y1234/Y1235) on different lysates with Rabbit anti-Phospho-Met (Y1234/Y1235) antibody (HA722765) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa starved for 16 hours then treated with 20ng/mL HGF for 10 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.