FOLR1 Recombinant Rabbit Monoclonal Antibody [PSH06-93]
cat.: HA722768
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH06-93 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FOLR1 aa 25-257. |
| Positive control: | HeLa cell lysate, JAR cell lysate, human ovary cancer tissue, HeLa. |
| Subcellular location: | Cell membrane, Apical cell membrane, Basolateral cell membrane, Secreted, Cytoplasmic vesicle, clathrin-coated vesicle, Endosome. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P15328 Human |
| Alternative names: | adult Adult folate binding protein Adult folate-binding protein FBP Folate Binding Protein Folate Receptor 1 Adult Folate receptor 1 Folate Receptor 1 Precursor Folate receptor adult Folate receptor alpha Folate receptor FOLR FOLR1 FOLR1_HUMAN FR alpha FR-alpha FRalpha KB cells FBP MOV18 Ovarian cancer associated antigen Ovarian tumor associated antigen Ovarian tumor associated antigen MOv18 Ovarian tumor-associated antigen MOv18 |
Images
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Fig1:
Western blot analysis of FOLR1 on different lysates with Rabbit anti-FOLR1 antibody (HA722768) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: JAR cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 38 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722768) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-FOLR1 antibody (HA722768) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722768) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of MDA-MB-231 (left, negative) and HeLa (right, positive) cells labeling FOLR1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722768, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.