FRA1 Recombinant Rabbit Monoclonal Antibody [PSH06-95]
cat.: HA722771
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-95 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FRA1 aa 1-271. |
| Positive control: | HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P15407 Human |
| Alternative names: | FOS L1 FOS like antigen 1 Fos related antigen 1 Fos-related antigen 1 FOSL 1 FOSL1 FOSL1 protein FOSL1_HUMAN FRA 1 FRA-1 |
Images
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Fig1:
Western blot analysis of FRA1 on different lysates with Rabbit anti-FRA1 antibody (HA722771) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa starved for 24 hours then add 100ng/mL TPA for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 35-40 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722771) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.