Phospho-STAT6 (Y641) Recombinant Rabbit Monoclonal Antibody [PSH06-98]
cat.: HA722776
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 94 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human p53 aa 611-660.. |
| Positive control: | Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P42226 Human | P52633 Mouse |
| Alternative names: | 12S1644 D12S1644 IL 4 STAT IL-4 Stat IL4 STAT Interleukin 4 Induced Interleukin 4 Induced Transcription Factor IL4 STAT Signal transducer and activator of transcription 6 Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 Signal transducer and activator of transcription 6, interleukin 4 induced STAT 6 STAT interleukin4 induced STAT, interleukin4 induced Stat6 STAT6_HUMAN STAT6B STAT6C Transcription factor IL 4 STAT |
Images
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Fig1:
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA722776) at 1/2,000 dilution. Lane 1: J774A.1 cell lysate Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722776) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.