HA722781

Aquaporin 5 Recombinant Rabbit Monoclonal Antibody [JE05-51]

cat.: HA722781

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, IF-Tissue, mIHC
Clonality:	Monoclonal
Clone number:	JE05-51

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 28 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Aquaporin 5 aa 225-265 / 265.
Positive control:	Human lung tissue lysate, Mouse lung tissue lysate, human stomach tissue, mouse lung tissue.
Subcellular location:	Apical cell membrane, Cell membrane, Cytoplasmic vesicle membrane.
Recommended Dilutions: WB IHC-P IF-Tissue mIHC	1:1,000-1:2,000 1:1,000 1:500 1:1,000
Uniprot #:	SwissProt: P55064 Human \| Q9WTY4 Mouse
Alternative names:	AQP 5 AQP-5 AQP5 AQP5_HUMAN Aquaporin 5 Aquaporin-5

Images

Fig1: Western blot analysis of Aquaporin 5 on different lysates with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution.

Lane 1: Human lung tissue lysate
Lane 2: Human liver tissue lysate (negative)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 25 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of Aquaporin 5 on different lysates with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/2,000 dilution.

Lane 1: Mouse lung tissue lysate
Lane 2: Mouse kidney tissue lysate (negative)
Lane 3: Mouse liver tissue lysate (negative)

Lysates/proteins at 40 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 25 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722781) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunofluorescence analysis of paraffin-embedded human stomach tissue labeling Aquaporin 5 with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722781, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722781) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722781) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722781) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue (negative) with Rabbit anti-Aquaporin 5 antibody (HA722781) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.