CRMP5 Recombinant Rabbit Monoclonal Antibody [JE34-69]
cat.: HA722782
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE34-69 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CRMP5 aa 515-564 / 564. |
| Positive control: | SH-SY5Y cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, Rat brain tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9BPU6 Human | Q9EQF6 Mouse | Q9JHU0 Rat |
| Alternative names: | Collapsin response mediator protein 5 CRAM CRMP 3 associated molecule CRMP 5 CRMP3 associated molecule Dihydropyrimidinase like 5 Dihydropyrimidinase related protein 5 Dihydropyrimidinase-related protein 5 DPYL5_HUMAN DPYS L5 DPYSL 5 DPYSL5 DRP 5 DRP5 FLJ45383 Ulip 6 Ulip6 ULIP6 protein UNC33-like phosphoprotein 6 |
Images
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Fig1:
Western blot analysis of CRMP5 on different lysates with Rabbit anti-CRMP5 antibody (HA722782) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722782) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
CRMP5 was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA722782 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722782 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: HA722782 IP in SH-SY5Y cell lysate Lane 3: Rabbit IgG instead of HA722782 in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.