Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE36-30 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 9 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within |
Positive control: | HepG2 cell lysate, HepG2 treated with 300ng/mL BFA for 24 hours cell lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P02654 Human |
Alternative names: | APO C1 Apo CI Apo-CIB Apo-CIB' APOC 1 ApoC I ApoC-IB ApoC-IB' APOC1 APOC1_HUMAN APOC1B Apolipoprotein C I Apolipoprotein C I variant I Apolipoprotein C-I Apolipoprotein C1 Apolipoprotein CI ApolipoproteinC I ApolipoproteinCI Truncated apolipoprotein C-I |
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Fig1:
Western blot analysis of Apolipoprotein CI / Apo-CI on different lysates with Rabbit anti-Apolipoprotein CI / Apo-CI antibody (HA722783) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HepG2 treated with 300ng/mL BFA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 9 kDa Observed band size: 9 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722783) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |