Phospho-CBL (Y774) Recombinant Rabbit Monoclonal Antibody [JE62-93]
cat.: HA722786
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE62-93 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Tyr774 of human CBL. |
| Positive control: | A549 starved for 2 hours then add 1μM sodium orthovanadate for 1 hour cell lysate, HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Cell projection, cilium, Golgi apparatus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P22681 Human |
| Alternative names: | 4732447J05Rik C CBL Cas Br M (murine) ecotropic retroviral transforming sequence Casitas B lineage lymphoma proto oncogene Casitas B-lineage lymphoma proto-oncogene CBL 2 cbl CBL_HUMAN CBL2 E3 ubiquitin protein ligase CBL E3 ubiquitin-protein ligase CBL Oncogene CBL2 Proto oncogene c CBL Proto-oncogene c-CBL RGD1561386 RING finger protein 55 RNF55 Signal transduction protein CBL |
Images
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Fig1:
Western blot analysis of Phospho-CBL (Y774) on different lysates with Rabbit anti-Phospho-CBL (Y774) antibody (HA722786) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 starved for 2 hours cell lysate Lane 3: A549 starved for 2 hours then add 1μM sodium orthovanadate for 1 hour cell lysate Lane 4: HeLa cell lysate Lane 5: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate Lane 6: A549 starved for 2 hours then add 1μM sodium orthovanadate for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lane 7: HeLa treated with 1mM sodium orthovanadate for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 10 µg/Lane. Predicted band size: 100 kDa Observed band size: 100-110 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722786) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.