Human CXCL13 Recombinant Rabbit Monoclonal Antibody [PSH07-07]
cat.: HA722794
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH07-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CXCL13 aa 23-109. |
| Positive control: | Recombinant Human CXCL13 protein (HA211065). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH07-05] to Human CXCL13 antibody (Capture) (HA722791) and recombinant Human CXCL13 protein (HA211065) as the standard. The reference range value is 12.3-3000pg/ml. |
| Uniprot #: | SwissProt: O43927 Human |
| Alternative names: | ANGIE ANGIE2 B cell attracting chemokine 1 B cell-attracting chemokine 1 B lymphocyte chemoattractant B-cell chemoattractant B-cell-attracting chemokine 1 B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) BCA-1 BCA1 BLC BLR1L C-X-C motif chemokine 13 Chemokine (C-X-C motif) ligand 13 Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) Chemokine, CXC motif, ligand 13 CXC chemokine BLC CXCL13 CXL13_HUMAN SCYB13 Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant) Small inducible cytokine B13 Small inducible cytokine subfamily B, member 13 Small-inducible cytokine B13 |
Images
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Fig1:
Sandwich ELISA analysis of Human CXCL13 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722791) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human CXCL13 protein (HA211065) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722794, Biotin, 0.3 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r and LPS for 24 hours. Interpolated concentration of native CXCL13 was measured in duplicate at different sample concentrations and interpolated from the CXCL13 standard curves. Undiluted samples were 20% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 10,653 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant. |
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Fig3:
Interpolated concentrations of spiked CXCL13 in human cell culture media samples. The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.