ATF-4 Recombinant Rabbit Monoclonal Antibody [PSH07-08]
cat.: HA722796
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH07-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATF-4 aa 1-300. |
| Positive control: | HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate, HeLa treated with 250μM CoCl2 for 6 hours cell lysate, NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate. |
| Subcellular location: | Nucleus, Nucleus speckle, Cytoplasm, Cell membrane, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P18848 Human | Q06507 Mouse | Q9ES19 Rat |
| Alternative names: | Activating transcription factor 4 ATF 4 ATF4 ATF4 protein ATF4_HUMAN cAMP-dependent transcription factor ATF-4 cAMP-responsive element-binding protein 2 CREB 2 CREB-2 CREB2 Cyclic AMP dependent transcription factor ATF 4 Cyclic AMP response element binding protein 2 Cyclic AMP-dependent transcription factor ATF-4 Cyclic AMP-responsive element-binding protein 2 DNA binding protein TAXREB67 DNA-binding protein TAXREB67 Tax Responsive Enhancer Element B67 Tax-responsive enhancer element-binding protein 67 TaxREB67 TXREB |
Images
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Fig1:
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA722796) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA722796) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
ATF-4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA722796 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722796 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input) Lane 2: HA722796 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Lane 3: Rabbit IgG instead of HA722796 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.