Phospho-Cofilin (S3) Recombinant Rabbit Monoclonal Antibody [PSH07-18]
cat.: HA722806
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-18 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser3 of human Cofilin aa 1-50. |
| Positive control: | COS-1 cell lysate, C6 cell lysate, Jurkat cell lysate, MCF7 cell lysate, A431 cell lysate. |
| Subcellular location: | Nucleus matrix, Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, lamellipodium membrane, lamellipodium, growth cone, axon. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P23528 Human | P45592 Rat |
| Alternative names: | 18 kDa phosphoprotein CFL 1 CFL CFL1 COF1_HUMAN Cofilin 1 Cofilin 1 non muscle Cofilin Cofilin non muscle isoform Cofilin-1 epididymis secretory protein Li 15 HEL-S-15 non-muscle isoform p18 |
Images
|
Fig1:
Western blot analysis of Phospho-Cofilin (S3) on different lysates with Rabbit anti-Phospho-Cofilin (S3) antibody (HA722806) at 1/2,000 dilution. Lane 1: COS-1 cell lysate Lane 2: C6 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: A431 cell lysate Lane 6: A431 cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722806) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.