Retinoic Acid Receptor gamma Recombinant Rabbit Monoclonal Antibody [PSH07-19]
cat.: HA722807
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH07-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RARG aa 155-454. |
| Positive control: | SK-Br-3 cell lysate, T-47D cell lysate, A375 cell lysate, HeLa cell lysate, MEF cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate, human skin tissue, mouse skin tissue, rat skin tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P13631 Human | P18911 Mouse Entrez Gene: 685072 Rat |
| Alternative names: | NR1B3 Nuclear receptor subfamily 1 group B member 3 RAR gamma RAR-gamma RARC Rarg RARG_HUMAN Retinoic acid receptor gamma 2 Retinoic acid receptor gamma Retinoic acid receptor, gamma |
Images
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Fig1:
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/2,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: T-47D cell lysate (20 µg/Lane) Lane 3: A375 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: MEF cell lysate (20 µg/Lane) Lane 6: Mouse skin tissue lysate (40 µg/Lane) Lane 7: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 58 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722807) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Retinoic Acid Receptor gamma on different lysates with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/5,000 dilution. Lane 1: Human RARG recombinant protein, 20ng/Lane Lane 2: Human RARA recombinant protein, 20ng/Lane Lane 3: Human RARB recombinant protein, 20ng/Lane Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722807) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722807) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722807) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Retinoic Acid Receptor gamma antibody (HA722807) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722807) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.