LATS1 / WARTS Recombinant Rabbit Monoclonal Antibody [PSH07-20]
cat.: HA722808
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Monkey
Applications: WB
Clonality: Monoclonal
Clone number: PSH07-20
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 127 kDa
Isotype: IgG
Immunogen: Recombinant protein within human LATS1 aa 151-600.
Positive control: HT-29 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, C2C12 cell lysate, COS-1 cell lysate.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, microtubule organizing center, spindle pole body.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O95835 Human | Q8BYR2 Mouse
Alternative names: h-warts Large tumor suppressor homolog 1 LATS large tumor suppressor homolog 1 LATS1 LATS1_HUMAN Serine threonine protein kinase LATS1 Serine/threonine-protein kinase LATS1 WARTS WARTS protein kinase wts
Images
HA722808_1.jpg Fig1: Western blot analysis of LATS1 / WARTS on different lysates with Rabbit anti-LATS1 / WARTS antibody (HA722808) at 1/1,000 dilution.

Lane 1: HT-29 cell lysate
Lane 2: K-562 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: HeLa cell lysate
Lane 5: C2C12 cell lysate
Lane 6: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 127 kDa
Observed band size: 127 kDa

Exposure time: 59 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722808) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.