| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LATS1 aa 151-600. |
| Positive control: | HT-29 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, C2C12 cell lysate, COS-1 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, microtubule organizing center, spindle pole body. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95835 Human | Q8BYR2 Mouse |
| Alternative names: | h-warts Large tumor suppressor homolog 1 LATS large tumor suppressor homolog 1 LATS1 LATS1_HUMAN Serine threonine protein kinase LATS1 Serine/threonine-protein kinase LATS1 WARTS WARTS protein kinase wts |
|
Fig1:
Western blot analysis of LATS1 / WARTS on different lysates with Rabbit anti-LATS1 / WARTS antibody (HA722808) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: K-562 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HeLa cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722808) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |