Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Monkey |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH07-20 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 127 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human LATS1 aa 151-600. |
Positive control: | HT-29 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, C2C12 cell lysate, COS-1 cell lysate. |
Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, microtubule organizing center, spindle pole body. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: O95835 Human | Q8BYR2 Mouse |
Alternative names: | h-warts Large tumor suppressor homolog 1 LATS large tumor suppressor homolog 1 LATS1 LATS1_HUMAN Serine threonine protein kinase LATS1 Serine/threonine-protein kinase LATS1 WARTS WARTS protein kinase wts |
Fig1:
Western blot analysis of LATS1 / WARTS on different lysates with Rabbit anti-LATS1 / WARTS antibody (HA722808) at 1/1,000 dilution. Lane 1: HT-29 cell lysate Lane 2: K-562 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HeLa cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722808) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |