Hormone sensitive lipase / HSL Recombinant Rabbit Monoclonal Antibody [PSH07-22]
cat.: HA722810
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH07-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 117 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HSL aa 931-980. |
| Positive control: | Jurkat cell lysate, SH-SY5Y cell lysate, Mouse brown adipose tissue lysate, Rat brown adipose tissue lysate, human breast tissue, mouse breast tissue, rat breast tissue. |
| Subcellular location: | Cell membrane, Membrane, caveola, Cytoplasm, cytosol, Lipid droplet. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:30,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q05469 Human | P54310 Mouse | P15304 Rat |
| Alternative names: | Hormone sensitive lipase Hormone sensitive lipase testicular isoform Hormone-sensitive lipase HSL LHS Lipase hormone sensitive LIPE LIPS_HUMAN |
Images
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Fig1:
Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 117 kDa Observed band size: 90 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Hormone sensitive lipase / HSL on different lysates with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/1,000 dilution. Lane 1: Human liver tissue lysate (negative) Lane 2: Mouse brown adipose tissue lysate Lane 3: Rat brown adipose tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 117 kDa Observed band size: 90 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722810) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat breast tissue with Rabbit anti-Hormone sensitive lipase / HSL antibody (HA722810) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722810) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Hormone sensitive lipase / HSL was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722810 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722810 at 1/3,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722810 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722810 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.