ERK8 Recombinant Rabbit Monoclonal Antibody [PSH07-23]
cat.: HA722811
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH07-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ERK8 aa 1-400. |
| Positive control: | Raji cell lysate, Mouse kidney tissue lysate, Mouse lung tissue lysate, Rat kidney tissue lysate, Rat lung tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cytosol, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q8TD08 Human | Q80Y86 Mouse | Q9Z2A6 Rat |
| Alternative names: | ERK 7 ERK 8 ERK-7 ERK-8 ERK7 ERK8 Extracellular regulated kinase 8 delta Extracellular signal regulated kinase 8 Extracellular signal-regulated kinase 7 Extracellular signal-regulated kinase 8 MAP kinase 15 MAPK 15 Mapk15 Mitogen activated protein kinase 15 Mitogen-activated protein kinase 15 MK15_HUMAN |
Images
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Fig1:
Western blot analysis of ERK8 on different lysates with Rabbit anti-ERK8 antibody (HA722811) at 1/2,000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (30 µg/Lane) Lane 3: Mouse lung tissue lysate (30 µg/Lane) Lane 4: Rat kidney tissue lysate (30 µg/Lane) Lane 5: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: Lane 1: 3 minutes; Lane 2-5: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722811) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ERK8 antibody (HA722811) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722811) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ERK8 antibody (HA722811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ERK8 antibody (HA722811) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722811) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.