MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody [PSH07-24]
cat.: HA722812
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH07-24 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MAP4K1 aa 301-500. |
| Positive control: | Jurkat cell lysate, Raji cell lysate, Ramos cell lysate, Daudi cell lysate, THP-1 cell lysate, human lymph node tissue, human spleen tissue. |
| Subcellular location: | Cytoplasm, membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q92918 Human |
| Alternative names: | Hematopoietic progenitor kinase 1 Hematopoietic progenitor kinase HPK1 Human Hematopoietic Progenitor Kinase M4K1_HUMAN Map4k1 MAPK/ERK kinase kinase kinase 1 MEK kinase kinase 1 MEKKK 1 Mitogen activated protein kinase kinase kinase kinase 1 Mitogen-activated protein kinase kinase kinase kinase 1 |
Images
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Fig1:
Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody (HA722812) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Raji cell lysate (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Predicted band size: 91 kDa Observed band size: 97 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722812) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-MAP4K1 / HPK1 antibody (HA722812) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722812) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MAP4K1 / HPK1 antibody (HA722812) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722812) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
MAP4K1 / HPK1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722812 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722812 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722812 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722812 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.