CYP11A1 Recombinant Rabbit Monoclonal Antibody [PSH07-26]
cat.: HA722814
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CYP11A1 aa 1-350. |
| Positive control: | HepG2 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse ovary tissue lysate, Mouse brain tissue lysate, Rat testis tissue lysate, Rat ovary tissue lysate. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P05108 Human | Q9QZ82 Mouse | P14137 Rat |
| Alternative names: | Cholesterol 20 22 desmolase Cholesterol desmolase Cholesterol monooxygenase (side chain cleaving) Cholesterol side chain cleavage enzyme Cholesterol side chain cleavage enzyme mitochondrial Cholesterol side-chain cleavage enzyme CP11A_HUMAN CYP11A CYP11A1 CYPXIA1 Cytochrome P450 11A1 Cytochrome P450 11A1 mitochondrial Cytochrome P450 family 11 subfamily A polypeptide 1 Cytochrome P450 subfamily XIA Cytochrome P450(scc) Cytochrome P450C11A1 mitochondrial P450SCC Steroid 20 22 lyase |
Images
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Fig1:
Western blot analysis of CYP11A1 on different lysates with Rabbit anti-CYP11A1 antibody (HA722814) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Caco-2 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Mouse ovary tissue lysate (40 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Rat testis tissue lysate (40 µg/Lane) Lane 9: Rat ovary tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 45 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722814) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.