Sp7 / Osterix Recombinant Rabbit Monoclonal Antibody [PSH07-29]
cat.: HA722817
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue, IHC-Fr
Clonality: Monoclonal
Clone number: PSH07-29
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 45 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Sp7 aa 1-300.
Positive control: Saos-2 cell lysates, human osteosarcoma tissue, mouse embryo tissue, rat embryo tissue, E14.5 mouse embryo tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Tissue
  IHC-Fr
1:2,000-1:5,000
1:200-1:1,000
1:500
1:200
Uniprot #: SwissProt: Q8TDD2 Human | Q8VI67 Mouse
Entrez Gene: 300260 Rat
Alternative names: MGC126598 Osterix Osx Sp 7 SP7 Sp7 transcription factor SP7_HUMAN Transcription factor Sp7 Zinc finger protein osterix
Images
HA722817_1.jpg Fig1: Western blot analysis of Sp7 / Osterix on Saos-2 cell lysates (30 µg/Lane) with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722817) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722817_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722817) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722817_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722817_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat embryo tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA722817_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded E14.5 mouse embryo tissue labeling Sp7 / Osterix with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/500 dilution and competitor's antibody at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722817, green) at 1/500 dilution and competitor's antibody at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA722817_6.jpg Fig6: Immunofluorescence analysis of frozen E14.5 mouse embryo tissue with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/200 dilution and competitor's antibody at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722817, green) at 1/200 dilution and competitor's antibody at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA722817_7.jpg Fig7: Western blot analysis of Sp7 / Osterix on different lysates with Rabbit anti-Sp7 / Osterix antibody (HA722817) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Rat brain tissue lysate (no heat)

Notice: no heat means the lysate is not boiled. We recommend using no heat or 1% SDS hot method to prepare lysate to improve signal intensity.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 45 kDa
Observed band size: 45 kDa

Exposure time: 3 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722817) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.