ERO1L Recombinant Rabbit Monoclonal Antibody [PSH07-30]
cat.: HA722818
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH07-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ERO1L aa 1-468. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, L6 cell lysate, Mouse placenta tissue lysate, human colon tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, membrane protein, Golgi apparatus lumen, Secreted, Cell projection, dendrite. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q96HE7 Human | Q8R180 Mouse | Q8R4A1 Rat |
| Alternative names: | Endoplasmic oxidoreductin 1 like protein Endoplasmic oxidoreductin-1-like protein Endoplasmic reticulum oxidoreductase 1 alpha Endoplasmic reticulum oxidoreductin 1-like ERO1 alpha ERO1 L ERO1 Lalpha ERO1 like protein alpha ERO1-alpha ERO1-L ERO1-L-alpha ERO1-like (S. cerevisiae) ERO1-like alpha ERO1-like protein alpha ERO1-like, S. cerevisiae, homolog of, alpha ERO1A ERO1A_HUMAN ERO1L ERO1LA Oxidoreductin 1 Lalpha Oxidoreductin-1-L-alpha PRO865 UNQ434 |
Images
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Fig1:
Western blot analysis of ERO1L on different lysates with Rabbit anti-ERO1L antibody (HA722818) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: L6 cell lysate (20 µg/Lane) Lane 6: Mouse placenta tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 60 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722818) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ERO1L antibody (HA722818) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722818) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.