IRG1 Recombinant Rabbit Monoclonal Antibody [PSH07-31]
cat.: HA722819
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH07-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse IRG1 aa 1-488. |
| Positive control: | RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate, J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate, mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate, mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate, RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: P54987 Mouse |
| Alternative names: | ACOD1 Aconitate decarboxylase 1 Aconitate decarboxylase CAD Cis aconitate decarboxylase Immune responsive gene 1 protein homolog Immune-responsive gene 1 protein homolog Immunoresponsive 1 homolog (mouse) Irg1 IRG1_HUMAN OC116 OPTB1 TCTIRC7 |
Images
|
Fig1:
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody (HA722819) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate Lane 5: mBMMC cell lysate Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate Lane 7: mBMSC cell lysate Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722819) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of RAW264.7 cells treated with 0.1μg/mL LPS for 6 hours labeling IRG1 with Rabbit anti-IRG1 antibody (HA722819) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IRG1 antibody (HA722819) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.