SLUG Recombinant Rabbit Monoclonal Antibody [PSH07-40]
cat.: HA722828
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH07-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SLUG aa 1-150. |
| Positive control: | U-87 MG cell lysate, SK-MEL-28 cell lysate, SK-MEL-28. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: O43623 Human |
| Alternative names: | MGC10182 Neural crest transcription factor Slug Protein snail homolog 2 Slug (chicken homolog) zinc finger protein Slug homolog zinc finger protein Slug zinc finger protein SLUGH SLUGH 1 SLUGH1 SNAI 2 Snai2 SNAI2_HUMAN Snail 2 Snail homolog 2 Snail2 WS 2D WS2D Zinc finger protein SLUG Zinc finger protein SNAI2 |
Images
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Fig1:
Western blot analysis of SLUG on different lysates with Rabbit anti-SLUG antibody (HA722828) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: SK-MEL-28 cell lysate Lane 4: PANC-1 cell lysate (low expression) Lysates/proteins at 30 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 2 minutes 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722828) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of SK-MEL-28 (positive) and PANC-1 (low expression) labeling SLUG with Rabbit anti-SLUG antibody (HA722828) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLUG antibody (HA722828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.