Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody [PSH07-45]
cat.: HA722833
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH07-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 309 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Von Willebrand Factor aa 734-1,283. |
| Positive control: | Human lung tissue lysate, Rat lung tissue lysate, human appendix tissue, human colon carcinoma tissue, human lung tissue, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IP |
1:5,000 1:500 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: P04275 Human | Q8CIZ8 Mouse | A0A8J8XVZ5 Rat |
| Alternative names: | Coagulation factor VIII Coagulation factor VIII VWF F8VWF Factor VIII related antigen von Willebrand antigen 2 von Willebrand antigen II Von Willebrand disease Von Willebrand factor precursor VWD vWF VWF_HUMAN |
Images
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Fig1:
Western blot analysis of Von Willebrand Factor on different lysates with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HEK-293 cell lysate (negative) Lane 2: Human lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 309 kDa Observed band size: 309 kDa Exposure time: Lane 1-3 (left): 25 seconds; Lane 1-3 (right): 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722833) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722833) at 1/500 dilution and competitor's antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunofluorescence analysis of paraffin-embedded human appendix tissue labeling Von Willebrand Factor with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722833, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig8:
Immunofluorescence analysis of paraffin-embedded rat lung tissue labeling Von Willebrand Factor with Rabbit anti-Von Willebrand Factor antibody (HA722833) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722833, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig9:
Von Willebrand Factor was immunoprecipitated from 0.2 mg rat lung tissue lysate with HA722833 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722833 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: rat lung tissue lysate (input) Lane 2: HA722833 IP in rat lung tissue lysate Lane 3: Rabbit IgG instead of HA722833 in rat lung tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.