Mono-Methyl Arginine (R*GG) Recombinant Rabbit Monoclonal Antibody [PSH07-52]
cat.: HA722840
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic mono-methyl arginine peptide library containing R*GG motifs. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Alternative names: | Arginine (mono methyl) Mono-Methyl Arginine (R*GG) |
Images
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Fig1:
Western blot analysis of Mono-Methyl Arginine (R*GG) on different lysates with Rabbit anti-Mono-Methyl Arginine (R*GG) antibody (HA722840) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 10nM Type I PRMT inhibitor MS023 for 48 hours cell lysate Lysates/proteins at 20 µg/Lane. Observed band size: Mutiple kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722840) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.