CXCL1 / GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH07-53]
cat.: HA722841
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH07-53 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CXCL1 aa 1-107. |
| Positive control: | HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P09341 Human |
| Alternative names: | C-X-C motif chemokine 1 Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) chemokine (C-X-C motif) ligand 1 CINC-1 CXCL1 Cytokine-induced neutrophil chemoattractant 1 Fibroblast secretory protein Fsp Gro 1 Gro A Gro GRO protein, alpha GRO-alpha(1-73) GRO-alpha(6-73) Gro1 GRO1 oncogene (melanoma growth stimulating activity, alpha) GRO1 oncogene (melanoma growth-stimulating activity) Gro1 oncogene GROa GROA_HUMAN Growth-regulated alpha protein KC KC chemokine, mouse, homolog of melanoma growth stimulatory activity alpha Melanoma growth stimulatory activity Melanoma growth stimulatory activity, alpha MGSA alpha MGSA MGSA-a N51 NAP-3 NAP3 Neutrophil-activating protein 3 Platelet-derived growth factor-inducible protein KC Scyb 1 Scyb1 Secretory protein N51 Small inducible cytokine subfamily B, member 1 |
Images
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Fig1:
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA722841) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate Lane 3: EA.hy926 cell lysate Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.