NrCAM Recombinant Rabbit Monoclonal Antibody [JE30-78]
cat.: HA722843
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE30-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 144 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NrCAM aa 821-970 / 1,304. |
| Positive control: | SH-SY5Y cell lysate, A549 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue lysates. |
| Subcellular location: | Cell membrane,Cell projection,,Secreted |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q92823 Human | Q810U4 Mouse | P97686 Rat |
| Alternative names: | Bravo hBravo Neuronal cell adhesion molecule Neuronal surface protein Bravo Ng CAM related Ng-CAM-related NgCAM related cell adhesion molecule NgCAM-related cell adhesion molecule Nr CAM Nr-CAM Nrcam NRCAM_HUMAN |
Images
|
Fig1:
Western blot analysis of NrCAM on different lysates with Rabbit anti-NrCAM antibody (HA722843) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 144 kDa Observed band size: 144 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NrCAM on human brain tissue lysates with Rabbit anti-NrCAM antibody (HA722843) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 144 kDa Observed band size: 144 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722843) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.