Phospho-PKC (S729) Recombinant Rabbit Monoclonal Antibody [JE77-54]
cat.: HA722846
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE77-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S729 of human PKC. |
| Positive control: | HeLa cell lysate, HeLa treated with 200nM TPA for 30 minutes cell lysate, C2C12 cell lysate, C2C12 treated with 200nM TPA for 30 minutes cell lysate, C6 cell lysate, C6 treated with 200nM TPA for 10 minutes cell lysate, human breast cancer tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell membrane, perinuclear region, Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P05129 Human | P05771 Human | P17252 Human | Q02156 Human | Q05513 Human | Q05655 Human |
| Alternative names: | KPCA_HUMAN PKC alpha PKC beta PKC delta PKC epsilon PKC gamma PKC zeta PKC-A PKC-alpha PKC2 PKCA PKCB PKCD PKCE PKCG PRKCA PRKCB PRKCB1 PRKCB2 PRKCD PRKCE PRKCG PRKCZ Protein kinase C alpha Protein kinase C alpha type Protein kinase C Protein kinase C beta Protein kinase C delta Protein kinase C epsilon Protein kinase C gamma Protein kinase C zeta |
Images
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Fig1:
Western blot analysis of Phospho-PKC (S729) on different lysates with Rabbit anti-Phospho-PKC (S729) antibody (HA722846) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 200nM TPA for 30 minutes cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 200nM TPA for 30 minutes cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 200nM TPA for 10 minutes cell lysate Lane 7: HeLa treated with 200nM TPA for 30 minutes cell lysate, then the membrane treated with Lambda PP for 1 hour Lane 8: C2C12 treated with 200nM TPA for 30 minutes cell lysate, then the membrane treated with Lambda PP for 1 hour Lane 9: C6 treated with 200nM TPA for 10 minutes cell lysate, then the membrane treated with Lambda PP for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722846) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Phospho-PKC (S729) antibody (HA722846) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722846) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.