Phospho-Cytosolic Phospholipase A2 (S505) Recombinant Rabbit Monoclonal Antibody [PSH07-56]
cat.: HA722849
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, Dot Blot |
| Clonality: | Monoclonal |
| Clone number: | PSH07-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser505 of human cPLA2 aa 491-540. |
| Positive control: | HeLa cell lysate, RAW264.7 cell lysates. |
| Subcellular location: | Cytoplasm, Golgi apparatus membrane, Nucleus envelope. |
| Recommended Dilutions:
WB Dot Blot |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P47712 Human | P47713 Mouse |
| Alternative names: | Calcium dependent phospholipid binding protein CPLA 2 cPLA2 alpha cPLA2 Cytosolic phospholipase A2 Cytosolic phospholipase A2 group IVA Lysophospholipase MGC126350 PA24A_HUMAN Phosphatidylcholine 2 acylhydrolase Phosphatidylcholine 2-acylhydrolase Phospholipase A2 group 4 A Phospholipase A2 group IVA (cytosolic calcium dependent) Phospholipase A2 group IVA PhospholipaseA2 PLA2G4 pla2g4a |
Images
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Fig1:
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722849) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722849) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Cytosolic Phospholipase A2 (negative) Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive) Proteins loading: 50ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 6 seconds; ECL: K1801. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.