BRCA2 Recombinant Rabbit Monoclonal Antibody [PSH07-55]
cat.: HA722850
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH07-55 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 384 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BRCA2 aa 3,219-3,418. |
| Positive control: | HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, MCF7. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P51587 Human |
| Alternative names: | BRCA 2 BRCA1/BRCA2 containing complex subunit 2 Brca2 BRCA2, DNA repair associated BRCA2_HUMAN BRCC 2 BRCC2 Breast and ovarian cancer susceptibility gene early onset breast and ovarian cancer susceptibility protein 2 Breast cancer 2 early onset Breast Cancer 2 tumor suppressor Breast cancer susceptibility protein BRCA2 Breast cancer type 2 susceptibility protein BROVCA2 FACD FAD 1 FAD FAD1 FANCB FANCD 1 FANCD FANCD1 FANCD1 gene Fanconi anemia complementation group D1 Fanconi anemia group D1 protein GLM3 mutant BRCA2 OTTHUMP00000018803 OTTHUMP00000042401 PNCA2 XRCC11 |
Images
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Fig1:
Western blot analysis of BRCA2 on different lysates with Rabbit anti-BRCA2 antibody (HA722850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 384 kDa Observed band size: 460 kDa Exposure time: 25 seconds; ECL: K1802; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of MCF7 cells labeling BRCA2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722850, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.